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smac diablo  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc smac diablo
    Smac Diablo, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smac+diablo/Smac%2FDiablo+Rabbit+mAb/pm41395703-84-85-109
    Average 93 stars, based on 47 article reviews
    smac diablo - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Ferroptosis triggers mitochondrial fragmentation via Drp1 activation
    Article Snippet: Membranes were blocked for at least 30 min in PBS containing 0.1% Tween 20 (PBST) (VWR) with 5% (w/v) dried milk powder (AppliChem). .. The membranes were then incubated overnight at 4 °C with primary antibodies against DRP1 (Cell signaling, 14647), PS616-DRP1 (CST, 3455), PS637-DRP1 (CST, 4867) PGAM5 (Sigma, HPA036978), MiD49 (Sigma Aldrich, PA5-46624), MiD51 (Sigma Aldrich, PA5-43348), ß-actin (Sigma Aldrich, A1978), α‐Cytc (BD, 556433), α‐GAPDH (Cell signaling, 97166S), β-tubulin (Cell signaling, 55559), Smac/Diablo (Cell signaling, D5S3R), α‐TOM70 (Santa Cruz, sc390545), α‐TIM23 (BD, BD611223), α‐Mfn2 (Abcam, ab56889) α‐Mitoferrin-1 (Proteintech, 26469-1-AP), α‐Frataxin (Abcam, ab113691), all diluted 1:1000 in PBST containing 5% bovine serum albumin (BSA) (Thermo Fisher). .. After washing with PBST, the membranes were incubated with horseradish peroxidase (HRP)-coupled secondary antibodies (Biotium) diluted 1:10,000 for at least 1 h at room temperature.

    Article Title: Ferroptosis triggers mitochondrial fragmentation via Drp1 activation.
    Article Snippet: Membranes were blocked for at least 30 min in PBS containing 0.1% Tween 20 (PBST) (VWR) with 5% (w/v) dried milk powder (AppliChem). .. The membranes were then incubated overnight at 4 °C with primary antibodies against DRP1 (Cell signaling, 14647), PS616-DRP1 (CST, 3455), PS637-DRP1 (CST, 4867) PGAM5 (Sigma, HPA036978), MiD49 (Sigma Aldrich, PA5-46624), MiD51 (Sigma Aldrich, PA5-43348), ß-actin (Sigma Aldrich, A1978), α‐Cytc (BD, 556433), α‐GAPDH (Cell signaling, 97166S), β-tubulin (Cell signaling, 55559), Smac/Diablo (Cell signaling, D5S3R), α‐TOM70 (Santa Cruz, sc390545), α‐TIM23 (BD, BD611223), α‐Mfn2 (Abcam, ab56889) α‐ Mitoferrin-1 (Proteintech, 26469-1-AP), α‐Frataxin (Abcam, ab113691), all diluted 1:1000 in PBST containing 5% bovine serum albumin (BSA) (Thermo Fisher). .. After washing with PBST, the membranes were incubated with horseradish peroxidase (HRP)-coupled secondary antibodies (Biotium) diluted 1:10,000 for at least 1 h at room temperature.

    Western Blot:

    Article Title: Sustained cancer‐relevant alternative RNA splicing events driven by PRMT5 in high‐risk neuroblastoma
    Article Snippet: .. The following antibodies were used in immunoblots: β‐actin (3700S, Cell Signaling Technology, Danvers, MA, USA, dilution 1 : 2000), E2F1 (3742S, Cell Signaling Technology, dilution 1 : 1000), symmetric dimethyl arginine (SDMe) (13222S, Cell Signaling Technology, dilution 1 : 1000), FLAG (clone M2, F1804, Sigma, St. Louis, MO, USA, 1 : 1000), GAPDH (clone 6C5, MAB374, Millipore, Burlington, MA, USA, 1 : 2000), Smac/DIABLO (2954S, Cell Signaling Technology, dilution 1 : 1000), BIM (BCL2L11) (2819, Cell Signaling Technology, dilution 1 : 1000), ACIN1 (A300‐999A, Bethyl, Montgomery, TX, USA, dilution 1 : 1000) CFLAR (AV00022‐QC0240, Sigma‐Aldrich, dilution 1 : 1000), MYCN (SC‐53993, Santa Cruz, Dallas, TX, USA, dilution 1 : 1000), PRMT5 (79998S, Cell Signaling Technology, dilution 1 : 1000), CPSF3 (SC‐393001, Santa Cruz, dilution 1 : 1000), CPSF4 (15023‐I‐AP, ProteinTech, Rosemont, IL, USA, dilution 1 : 1000), SRSF1 (SC‐33652, Santa Cruz, dilution 1 : 1000), SRSF3 (51039S, Cell Signaling Technology, dilution 1 : 1000), SRSF4 (H303‐670A, Bethyl, dilution 1 : 1000), HNRNPA3 (25142‐I‐AP, ProteinTech, dilution 1 : 1000), HNRNPM (TA803154, ORIGENE, Rockville, MD, USA, dilution 1 : 1000). .. Cells were seeded in 96‐well plates and then maintained in the presence of vehicle or increasing doses of T1‐44 and T1‐68 (0.001 n m –100 μ m ) for 6 days before the addition of 10 μL of thiazolyl blue tetrazolium bromide (MTT) (Sigma‐Aldrich).

    Centrifugation:

    Article Title: A dual-purification system to isolate mitochondrial subpopulations.
    Article Snippet: .. After centrifugation, supernatant was carefully aspirated, pellets were resuspended in their respective buffers with PMSF and without Proteinase K. All samples were then boiled for 5 minutes prior to running on SDS-PAGE following standard procedures; denoted antibodies: Tomm20 (Cell Signaling Technologies, cat Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t no. 42406), Smac/Diablo (Cell Signaling Technologies, cat no. 2954), HtrA2/Omi (Cell Signaling Technologies, cat. no. 9745), Hsp60 (Cell Signaling Technologies, cat. no. 12165). ..

    SDS Page:

    Article Title: A dual-purification system to isolate mitochondrial subpopulations.
    Article Snippet: .. After centrifugation, supernatant was carefully aspirated, pellets were resuspended in their respective buffers with PMSF and without Proteinase K. All samples were then boiled for 5 minutes prior to running on SDS-PAGE following standard procedures; denoted antibodies: Tomm20 (Cell Signaling Technologies, cat Jo ur na l o f C el l S ci en ce • A cc ep te d m an us cr ip t no. 42406), Smac/Diablo (Cell Signaling Technologies, cat no. 2954), HtrA2/Omi (Cell Signaling Technologies, cat. no. 9745), Hsp60 (Cell Signaling Technologies, cat. no. 12165). ..



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